RESUMO
PURPOSE: To investigate the efficacy of intralesional 20% aspirin injection for treatment of experimental peritoneal endometriosis. Methods: Peritoneal endometriosis was experimentally induced in forty adult nulligravid female rabbits. On day 30 post-endometriosis induction, rabbits were randomly divided to assess early (10 days) and late (20 days) effects of intralesional injection of physiological saline solution (control groups) in comparison to intralesional injection of 20% bicarbonate aspirin solution (experimental groups) as follows: control group 1 (10 days, n=10); control group 2 (20 days, n=10); experimental group 3 (10 days, n=10); experimental group 4 (20 days, n=10). Resected tissues, including endometriosis foci, were qualitatively (general morphology and signs of inflammatory cells infiltrate, necrosis and apoptosis) and quantitatively (remaining endometriosis area) assessed by histopathological analysis. Results: Extensive necrosis, hemorrhage, apoptosis, and fibrosis were observed in the experimental groups 3 and 4. Groups 1 and 2 presented typical endometrial tissue cysts, respectively. Groups 3 and 4 showed sparse endometrial tissue foci and no endometrial tissue, respectively. Quantitative analysis revealed that aspirin-treated groups 3 and 4 had significantly (p<0.05) smaller remaining endometriosis area, compared to control groups 1 and 2. Conclusion: Intralesional 20% aspirin injection caused total destruction of peritoneal endometriosis foci in rabbits.
Assuntos
Animais , Feminino , Coelhos , Doenças Peritoneais/tratamento farmacológico , Injeções Intralesionais , Aspirina/administração & dosagem , Inibidores de Ciclo-Oxigenase/administração & dosagem , Endometriose/tratamento farmacológico , Doenças Peritoneais/patologia , Peritônio/efeitos dos fármacos , Peritônio/patologia , Fatores de Tempo , Reprodutibilidade dos Testes , Resultado do Tratamento , Apoptose , Modelos Animais de Doenças , Endometriose/patologia , Endométrio/efeitos dos fármacos , Endométrio/patologia , Anti-Inflamatórios/administração & dosagemRESUMO
PURPOSE: To evaluate the effects of copaiba oil administered by different routes on survival of mices subjected to cecal ligation and puncture. METHODS: Thirty two mice were distributed into four study groups (N=8): Sham group: normal standard animals; Control group: submitted a cecal ligation and puncture (CLP); Gavage group: submitted a CLP, and treat with copaiba oil by gavage; and Subcutaneous group: submitted a CLP, and treat with copaiba oil by subcutaneous injection. After the death of the histological analysis were performed. The Kaplan-Meier curves of surviving time were realized. RESULTS: All animals that received copaiba, regardless of the route used, survived longer when compared to the control group (p<0.0001), whereas the survival time ranged from 20 hours for the control group up to 32 hours for the animals of gavage group and 52 for subcutaneous group. The animals that received gavage copaiba lived about and about 20 hours unless the subcutaneous group (p=0.0042). There was no statistical difference when compared the intensity of inflammatory response (p>0.05) CONCLUSION: Prophylactic subcutaneous administration of copaiba in mice subjected to severe sepsis by cecal ligation and puncture, resulted in a survival time higher than non-use or use of this oil by gavage. .
Assuntos
Animais , Masculino , Camundongos , Bálsamos/administração & dosagem , Sepse/tratamento farmacológico , Bálsamos/uso terapêutico , Ceco/cirurgia , Injeções Subcutâneas/métodos , Intubação Gastrointestinal/métodos , Ligadura , Pulmão/patologia , Modelos Animais , Necrose/patologia , Punções , Doenças Peritoneais/tratamento farmacológico , Distribuição Aleatória , Análise de Sobrevida , Fatores de TempoRESUMO
Disseminated tuberculosis in HIV infection involves multiple organs. Pulmonary and lymph node involvement are the commonest form of tuberculosis in HIV infection [1, 2]. Other forms of tuberculosis in the absence of lung and lymph node involvement are rare. Various forms of abdominal [3, 4] and neurological [5, 6] tubercular involvement in HIV infection have been reported. But tuberculosis presenting simultaneously with mesenteric and brain abscess has not been reported yet. We report a case of disseminated tuberculosis presenting as mesenteric and cerebral abscess in a HIV case without involving lung and lymph nodes. Bone marrow smears and fine needle aspiration cytology (FNAC) from mesenteric lesion were positive for acid fast bacilli (AFB) and the diagnosis of tuberculosis was confirmed by positive polymerase chain reaction (PCR). He responded well to treatment with anti tubercular drugs.
Assuntos
Adulto , Humanos , Masculino , Infecções Oportunistas Relacionadas com a AIDS/complicações , Abscesso Encefálico/etiologia , Mesentério/microbiologia , Doenças Peritoneais/etiologia , Tuberculose/complicações , Abscesso , Infecções Oportunistas Relacionadas com a AIDS/diagnóstico , Infecções Oportunistas Relacionadas com a AIDS/tratamento farmacológico , Antituberculosos/uso terapêutico , Biópsia por Agulha Fina , Abscesso Encefálico/diagnóstico , Abscesso Encefálico/tratamento farmacológico , Mesentério/patologia , Reação em Cadeia da Polimerase , Doenças Peritoneais/diagnóstico , Doenças Peritoneais/tratamento farmacológico , Tomografia Computadorizada por Raios X , Tuberculose/diagnóstico , Tuberculose/tratamento farmacológicoRESUMO
BACKGROUND: Adhesion formation after peritoneal surgery is a major cause of postoperative bowel obstruction, infertility, and chronic pelvic pain. In this study, we compared the possible individual effects of phosphatidylcholine (PC), Seprafilm® II, and tissue plasminogen activator (t-PA) and the combined effects of phosphatidylcholine and t-PA on postoperative adhesion formation in a rat surgical model. MATERIALS AND METHODS: A total of 50 Wistar male rats underwent median laparotomy and standardized abrasion of the visceral and parietal peritoneum. phosphatidylcholine, Seprafilm II, and t-PA alone and phosphatidylcholine and t-PA in combination were applied intraperitoneally at the end of the surgical procedure. Seven days after surgery, a relaparotomy was performed for adhesion grading and histopathological examination. RESULTS: A comparison of adhesion stages demonstrated a significant difference between the control group and the study groups (p<0.001). The adhesion grade of the combined treatment group was statistically different from that of the other groups (p<0.05). In the t-PA group and the combined group, six and two rats, respectively, developed hematomas locally on the cecum. CONCLUSIONS: PC, t-PA, and Seprafilm II used individually reduced the adhesion grade. The t-PA and phosphatidylcholine combination was most effective in reducing adhesion formation. On the other hand, usage of t-PA alone or in combination may increase risk of bleeding. More detailed studies are needed, and future studies on the efficacy of a material for decreasing adhesion formation should include a comparison of several control materials in the same model.
Assuntos
Animais , Masculino , Ratos , Fibrinolíticos/uso terapêutico , Ácido Hialurônico/uso terapêutico , Doenças Peritoneais/tratamento farmacológico , Fosfatidilcolinas/uso terapêutico , Complicações Pós-Operatórias/tratamento farmacológico , Ativador de Plasminogênio Tecidual/uso terapêutico , Modelos Animais de Doenças , Combinação de Medicamentos , Avaliação Pré-Clínica de Medicamentos , Quimioterapia Combinada , Doenças Peritoneais/prevenção & controle , Complicações Pós-Operatórias/prevenção & controle , Ratos Wistar , Aderências Teciduais/tratamento farmacológico , Aderências Teciduais/prevenção & controleRESUMO
Isolated omental hydatid cyst is a very rare clinical entity. Here, such a case has been presented where abdominal cystic mass came out as isolated hydatid cyst in the greater omentum.
Assuntos
Animais , Equinococose/tratamento farmacológico , Feminino , Humanos , Pessoa de Meia-Idade , Omento/parasitologia , Doenças Peritoneais/tratamento farmacológico , Doenças Raras , Resultado do TratamentoRESUMO
We present a case of a young man who presented with fever and nonspecific epigastric symptoms and fluid collection in the lesser omental sac, which was proved to be of tubercular etiology.
Assuntos
Adulto , Antituberculosos/uso terapêutico , Humanos , Masculino , Omento/efeitos dos fármacos , Doenças Peritoneais/tratamento farmacológico , Tuberculose/complicaçõesRESUMO
Se operaron 15 perros utilizados como patrones, a los cuales se les provocaron adherencias intraperitoneales posoperatorias por el método de Thomas, modificado por nosotros. Estos perros fueron divididos en 3 grupos: IA: Se reintervinieron a las 4 semanas; IB: Se reintervinieron a las 8 semanas; IC: La reintervención se efectuó a las 12 semanas. Se utilizó como método profiláctico el uso intraperitoneal de acetato de hidrocortisona a las dosis de 2,5 mg por Kg de peso. Como testigo se hizo otro grupo de 15 perros, divididos en igual forma que el patrón, pero fueron medicamentados. Se exponen los resultados